human ES and iPSC Supplement (RMMS-00560193)
Cat. No.: RMMS-00560193
Category: Supplements
Research Area: Cell Line Development, Disease Modeling, Stem Cell Biology
INQUIRY
25 mL
Defined supplement for improving survival of human ES and iPS cells in single-cell workflows
Product Features
| Overview | Generate clonal human pluripotent stem cell (hPSC) lines that maintain their genomic integrity and downstream differentiation potential with this supplement. By using this product, you can increase the cloning efficiency and survival of human embryonic stem (ES) and induced pluripotent stem (iPS) cells under high-stress conditions, including seeding at low or high densities. For your gene-editing workflows, add this to improve ES and iPS cell survival following electroporation and during clonal deposition (see data below). It also facilitates the survival and expansion of clonal lines generated by low-density seeding (<25 cells/cm2) or by FACS-sorting cells at 1 cell/well, without requiring single-cell adaptation. You can also use this supplement to increase survival of single cells plated at higher densities, including under stressful conditions such as the post-thaw recovery of ES and iPS cell lines and when creating monolayers ahead of downstream differentiation. Defined and serum-free, this supplement is compatible for use with ES and iPS cell maintenance media, as well as your choice of cell culture matrix. |
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| Species | Human |
| Cell Type | Pluripotent Stem Cells |
| Applications | Cell Culture |
| Formulation | Serum-Free |
Storage & Shipping
| Storage | -20°C or colder |
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| Shipping | Shipped on gel packs. |
For research use only. Not for clinical use.