Human FABP4 ELISA Kit (RMEK-0152592)
Cat. No.: RMEK-0152592
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human FABP4 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FABP4 protein in cell culture extracts, cell culture supernatant, serum, tissue extracts, cit plasma, edta plasma, and hep plasma. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-9-2.8%; Inter-Assay-Serum-3-8.6% |
| Sensitivity | 2.7 pg/mL |
| Range | 4.69 pg/mL - 300 pg/mL |
| Sample Type | Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts |
| Recovery | Cell culture supernatant-107-105% - 111%; Serum-105-101% - 107%; Cell culture extracts-103-97% - 108%; Tissue Extracts-104-98% - 108%; Hep Plasma-106-104% - 108%; EDTA Plasma-105-104% - 106%; Cit plasma-108-107% - 110% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 2.7 pg/mL; Range: 4.69 pg/mL - 300 pg/mL; Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | FABP4 |
|---|---|
| UniProt ID | P15090 |
| Function | Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. |
| Cellular Localization | Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export. |
| Domain | Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
