Human FAS ELISA Kit (RMEK-0152596)
Cat. No.: RMEK-0152596
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human FAS ELISA kit has been re-developed. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Media-8-3.8%; Inter-Assay-Media-3-9.5% |
| Sensitivity | 26 pg/mL |
| Range | 46.88 pg/mL - 3000 pg/mL |
| Sample Type | Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Cell culture supernatant-97-91% - 108%; Serum-97-88% - 107%; Cell culture media-100-94% - 105%; Hep Plasma-105-98% - 111%; EDTA Plasma-92-81% - 102%; Cit plasma-98-90% - 105% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 26 pg/mL; Range: 46.88 pg/mL - 3000 pg/mL; Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | FAS |
|---|---|
| UniProt ID | P25445 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). |
| Cellular Localization | Secreted and Cell membrane. |
| Domain | Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins. |
| Involvement in Disease | Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A); also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
