Human FGF2 ELISA Kit (RMEK-0152603)
Cat. No.: RMEK-0152603
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human FGF2 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGF2 protein in cit plasma, hep plasma, serum, cell culture extracts, tissue extracts, and cell culture supernatant. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Extract-8-3.7%; Inter-Assay-Extract-3-1.8% |
| Sensitivity | 4 pg/mL |
| Range | 15.625 pg/mL - 2500 pg/mL |
| Sample Type | Cell culture extracts, Cell culture supernatant, Cit plasma, Hep Plasma, Serum, Tissue Extracts |
| Recovery | Cell culture supernatant-95-84% - 104%; Serum-103-101% - 106%; Cell culture extracts-117-103% - 125%; Cell culture media-110-101% - 115%; Hep Plasma-95-89% - 105%; Cit plasma-95-92% - 100% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 4 pg/mL; Range: 15.625 pg/mL - 2500 pg/mL; Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, Hep Plasma, Serum, Tissue Extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | FGF2 |
|---|---|
| UniProt ID | P09038 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis. |
| Cellular Localization | Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57. |
| Post-transcriptional Modifications | Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
