Human Fibronectin ELISA Kit, Fluorescent (RMEK-0152619)
Cat. No.: RMEK-0152619
Category: ELISA Kits
INQUIRY
1 x 96 tests
Fibronectin in vitro ELISA kit is designed for the quantitative measurement of Fibronectin protein in human serum, plasma, milk, cell culture supernatant, and cell and tissue extract samples. The ELISA Kit employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
Product Features
| Species Reactivity | Human, Rhesus monkey |
|---|---|
| Detection Method | Fluorescent |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-3-5%; Inter-Assay-Serum-5-8.3% |
| Sensitivity | 26 pg/mL |
| Range | 31 pg/mL - 32000 pg/mL |
| Sample Type | Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Milk, Serum, Tissue Extracts |
| Recovery | Cell culture supernatant-102-90% - 117%; Milk-102-91% - 111%; Serum-93-87% - 102%; Cell culture extracts-102-90% - 117%; Tissue Extracts-90-80% - 131%; EDTA Plasma-86-81% - 94%; Cit plasma-99-87% - 120% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 26 pg/mL; Range: 31 pg/mL - 32000 pg/mL; Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Milk, Serum, Tissue Extracts; Detection method: Fluorescent; Assay type: Sandwich (quantitative); Reacts with: Human, Rhesus monkey |
Target Information
| Target Symbol | FN |
|---|---|
| UniProt ID | P02751 |
| Function | Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. |
| Development Stage | Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. |
| Post-transcriptional Modifications | Sulfated.It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).Phosphorylated by FAM20C in the extracellular medium.Proteolytic processing produces the C-terminal NC1 peptide, anastellin. |
| Involvement in Disease | Glomerulopathy with fibronectin deposits 2 |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
