Human FLRG ELISA Kit (RMEK-0152622)
Cat. No.: RMEK-0152622
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human FLRG ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FLRG protein in edta plasma, hep plasma, milk, serum, urine, and cell culture supernatant. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-4.7%; Inter-Assay-Serum-3-6.4% |
| Sensitivity | 8.2 pg/mL |
| Range | 31.25 pg/mL - 2000 pg/mL |
| Sample Type | Cell culture supernatant, EDTA Plasma, Hep Plasma, Milk, Serum, Urine |
| Recovery | Cell culture supernatant-107-104% - 111%; Milk-103-98% - 109%; Urine-98-94% - 109%; Serum-106-105% - 109%; Hep Plasma-101-99% - 105%; EDTA Plasma-104-102% - 106% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 8.2 pg/mL; Range: 31.25 pg/mL - 2000 pg/mL; Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Milk, Serum, Urine; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | FLRG |
|---|---|
| UniProt ID | O95633 |
| Function | Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10. |
| Cellular Localization | Secreted and Nucleus. Although alternative initiation has been demonstrated and resulted in different localization, the major source of nuclear FSTL3 appears not to depend on translation initiation at Met-27 according to. |
| Involvement in Disease | A chromosomal aberration involving FSTL3 is found in a case of B-cell chronic lymphocytic leukemia. Translocation t(11; 19)(q13; p13) with CCDN1. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
