Human GATA3 ELISA Kit (RMEK-0152632)
Cat. No.: RMEK-0152632
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human GATA3 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GATA3 protein in cell culture extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-T47D Cells-9-3.2%; Inter-Assay-T47D Cells-3-4.39% |
| Sensitivity | 7.29 pg/mL |
| Range | 62.5 pg/mL - 4000 pg/mL |
| Sample Type | Cell culture extracts |
| Recovery | Cell culture extracts-107-106% - 108% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 7.29 pg/mL; Range: 62.5 pg/mL - 4000 pg/mL; Sample type: Cell culture extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | GATA3 |
|---|---|
| UniProt ID | P23771 |
| Biomarker of SCs/CSCs | Triple Negative Breast Cancer |
| Function | Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression (By similarity). Coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL33. Upon tissue injury, acts downstream of IL33 signaling to drive differentiation of inflammation-resolving alternatively activated macrophages. |
| Cellular Localization | Nucleus. |
| Domain | Binds DNA via the 2 GATA-type zinc fingers. Each zinc finger may bind either adjacent sites in a palindromic motif, or a different DNA molecule allowing looping and long-range gene regulation. The YxKxHxxxRP motif is critical for DNA-binding and function. |
| Involvement in Disease | Hypoparathyroidism, sensorineural deafness, and renal disease (HDR): A disease characterized by steroid-resistant nephrosis with progressive renal failure, hypoparathyroidism, sensorineural deafness, and renal dysplasia. The disease is caused by variants affecting the gene represented in this entry. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
