Human GATA4 ELISA Kit (RMEK-0152633)
Cat. No.: RMEK-0152633
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human GATA4 kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GATA4 protein in human cell and tissue extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Extract-8-8%; Inter-Assay-Extract-3-9.4% |
| Sensitivity | 4.02 pg/mL |
| Range | 15.625 pg/mL - 1000 pg/mL |
| Sample Type | Cell Lysate, Tissue Extracts |
| Recovery | Tissue Extracts-105-97% - 111%; Cell Lysate-100-96% - 106% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 4.02 pg/mL; Range: 15.625 pg/mL - 1000 pg/mL; Sample type: Cell Lysate, Tissue Extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | GATA4 |
|---|---|
| UniProt ID | P43694 |
| Function | Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement. Required during testicular development. May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase. |
| Cellular Localization | Nucleus. |
| Post-transcriptional Modifications | Methylation at Lys-300 attenuates transcriptional activity. |
| Involvement in Disease | Atrial septal defect 2Ventricular septal defect 1Tetralogy of FallotAtrioventricular septal defect 4Testicular anomalies with or without congenital heart diseaseGATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
