Human GCSF Receptor ELISA Kit (RMEK-0152635)
Cat. No.: RMEK-0152635
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human GCSF Receptor ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human GCSF Receptor in human serum, plasma (EDTA), cell culture supernatant, and cell and tissue extract. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Extract-8-2.2%; Inter-Assay-Extract-4-3.4% |
| Sensitivity | 9.45 pg/mL |
| Range | 28 pg/mL - 1800 pg/mL |
| Sample Type | Cell culture supernatant, Cell Lysate, EDTA Plasma, Serum |
| Recovery | Cell culture supernatant-102-100% - 104%; Serum-93-92% - 96%; Cell Lysate-114-113% - 115%; EDTA Plasma-92-92% - 94% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 9.45 pg/mL; Range: 28 pg/mL - 1800 pg/mL; Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | GCSF Receptor |
|---|---|
| UniProt ID | P09919 |
| Function | Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface. |
| Cellular Localization | Secreted and Cell membrane. |
| Domain | The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.The box 1 motif is required for JAK interaction and/or activation. |
| Involvement in Disease | Hereditary neutrophilia |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
