Human HDAC4 ELISA Kit (RMEK-0152662)
Cat. No.: RMEK-0152662
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human HDAC4 kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HDAC4 protein in human cell extract. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Extract-8-4.6%; Inter-Assay-Extract-3-5% |
| Sensitivity | 94.776 pg/mL |
| Range | 312.5 pg/mL - 20000 pg/mL |
| Sample Type | Cell Lysate |
| Recovery | Cell Lysate-85-83% - 90% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 94.776 pg/mL; Range: 312.5 pg/mL - 20000 pg/mL; Sample type: Cell Lysate; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | HDAC4 |
|---|---|
| UniProt ID | P56524 |
| Biomarker of SCs/CSCs | Dukes Type C Colon Cancer |
| Function | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. |
| Cellular Localization | Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation. |
| Domain | The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. |
| Post-transcriptional Modifications | Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3.Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4. |
| Involvement in Disease | Defects in HDAC4 are the cause of brachydactyly-mental retardation syndrome (BDMR). A syndrome resembling the physical anomalies found in Albright hereditary osteodystrophy. Common features are mild facial dysmorphism, congenital heart defects, distinct brachydactyly type E, mental retardation, developmental delay, seizures, autism spectrum disorder, and stocky build. Soft tissue ossification is absent, and there are no abnormalities in parathyroid hormone or calcium metabolism. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
