Human IgM ELISA Kit (RMEK-0152688)
Cat. No.: RMEK-0152688
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human IgM ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgM protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, saliva, and serum. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Plasma-8-4.9%; Inter-Assay-Plasma-3-5.8% |
| Sensitivity | 50 pg/mL |
| Range | 390.63 pg/mL - 25000 pg/mL |
| Sample Type | Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum |
| Recovery | Cell culture supernatant-100-94% - 105%; Saliva-101-94% - 110%; Milk-96-90% - 101%; Serum-96-93% - 98%; Hep Plasma-98-97% - 100%; EDTA Plasma-102-101% - 103%; Cit plasma-108-104% - 114% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 50 pg/mL; Range: 390.63 pg/mL - 25000 pg/mL; Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | IgM |
|---|---|
| UniProt ID | P0DOX6 |
| Function | Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen. IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The membrane-bound form is found in the majority of normal B cells alongside with IgD. Membrane-bound IgM induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. It may cause death of cells by apoptosis. It is also found in soluble form, which represents about 30% of the total serum immunoglobulins where it is found almost exclusively as a homopentamer. After the antigen binds to the B cell receptor, the secreted form is secreted in large amounts (, PubMed:16895553). |
| Cellular Localization | Secreted. Cell membrane. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
