Human Induced Pluripotent Stem (IPS) Cells [201B7] (RMCL-00260235)
Cat. No.: RMCL-00260235
Category: Cell Lines
INQUIRY
1 vial
Human Induced Pluripotent Stem Cells (iPSCs) were derived at Kyoto University from dermal fibroblasts obtained from a healthy donor. Fibroblasts were reprogrammed by the expression of OCT4, SOX2, KLF4, and MYC using retroviral transduction. This cell line is one of the pioneer lines from the Yamanaka laboratory and serves as a "normal" control for designed experiments.
Product Features
| BSL | BSL-2 |
|---|---|
| Product Format | Frozen |
| Applications | Stem cell research |
| Product Type | Human cells |
| Organism | Homo sapiens, human |
| Tissue | Skin |
| Cell Type | iPSC |
| Disease | Normal |
| STR Profiling | Confirmed by STR analysis and karyotype by G-banding |
| Reprogramming Method | Retroviral expression of OCT4, SOX2, KLF4, and MYC genes |
QC
| Bacterial & Fungal Testing | Not detected |
|---|---|
| Mycoplasma Contamination | Not detected |
Doner Information
| Gender | Female |
|---|---|
| Age | 36 years |
| Ethnicity | White |
Storage & Shipping
| Storage Conditions | Vapor phase of liquid nitrogen |
|---|---|
| Unpacking and Storage Instructions | Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Handling procedure | Pluripotent Stem Cell SFM XF/FF Basement Membrane Gel, To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If, upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C will result in loss of viability. Important: This product will gel in 15 to 30 minutes above 15°C. Keep This product and labware on ice at all times to prevent the matrix from gelling prematurely. Calculate the appropriate This product volume per plate based on concentration and usage. The concentration of This product is found on the product label. Preparation of Culture Thaw This product in the refrigerator (2°C to 8°C), in ice, overnight. Dilute the thawed This product to 150 µg/mL in cold DMEM: F-12 on ice and mix well. Immediately coat each 6 cm dish with 2 mL diluted This product. Swirl dish gently to ensure that the entire dish is evenly covered. Leave the coated dishes at 37°C for one hour. Aspirate the coating solution and immediately plate the cells. It is critical that the coating does not dry out. Protocol for Coating Plates One Hour Prior to Thawing the iPS Cells - Prepare coated plates as described. 30 Minutes Prior to Handling Cells - Pre-warm Pluripotent Stem Cell SFM XF/FF at 37°C for at least 30 minutes before adding to cells. If using ROCK Inhibitor Y27632, prepare stem cell culture medium supplemented with final concentration of 10 μM ROCK Inhibitor Y27632. Stem cell culture medium with ROCK inhibitor must be used immediately. |
For research use only. Not for clinical use.
