Human Interferon gamma ELISA Kit (RMEK-0152749)
Cat. No.: RMEK-0152749
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human Interferon gamma kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Interferon gamma protein in human serum, plasma, saliva and cell culture supernatant. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Supernatant-8-7.4%; Inter-Assay-Supernatant-3-11.38% |
| Sensitivity | 3.428 pg/mL |
| Range | 7.813 pg/mL - 500 pg/mL |
| Sample Type | Cell culture media, Cell culture supernatant, EDTA Plasma, Hep Plasma, Saliva, Serum |
| Recovery | Cell culture supernatant-104-97% - 115%; Serum-101-96% - 104%; Hep Plasma-103-93% - 114%; EDTA Plasma-100-93% - 106%; Cit plasma-101-95% - 109% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 3.428 pg/mL; Range: 7.813 pg/mL - 500 pg/mL; Sample type: Cell culture media, Cell culture supernatant, EDTA Plasma, Hep Plasma, Saliva, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | IFNG |
|---|---|
| UniProt ID | P01579 |
| Function | Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. |
| Cellular Localization | Secreted. |
| Post-transcriptional Modifications | Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. |
| Involvement in Disease | In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA). AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
