Human Leptin ELISA Kit (RMEK-0152775)
Cat. No.: RMEK-0152775
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human Leptin ELISA kit has been re-developed. The technology. Quantitate human Leptin with 4. 65 pg/mL sensitivity.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-5.1%; Inter-Assay-Serum-3-3.5% |
| Sensitivity | 4.65 pg/mL |
| Range | 15.63 pg/mL - 1000 pg/mL |
| Sample Type | Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Plasma, Serum |
| Recovery | Serum-91-90% - 93%; Cell culture media-102-101% - 104%; Hep Plasma-98-88% - 105%; EDTA Plasma-82-80% - 87%; Cit plasma-113-107% - 123% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 4.65 pg/mL; Range: 15.63 pg/mL - 1000 pg/mL; Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | LEP |
|---|---|
| UniProt ID | P41159 |
| Function | May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. |
| Cellular Localization | Secreted. |
| Involvement in Disease | Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.