Human N-Cadherin ELISA Kit (RMEK-0152811)
Cat. No.: RMEK-0152811
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human N-Cadherin ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of N-Cadherin protein in edta plasma, hep plasma, cell culture extracts, serum, and cell culture supernatant. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Cow, Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-4.4%; Inter-Assay-Serum-3-9.8% |
| Sensitivity | 41 pg/mL |
| Range | 156.25 pg/mL - 10000 pg/mL |
| Sample Type | Cell culture extracts, Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Cell culture supernatant-92-81% - 102%; Serum-114-113% - 115%; Cell culture extracts-97-84% - 108%; Hep Plasma-92-89% - 95%; EDTA Plasma-108-106% - 109% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 41 pg/mL; Range: 156.25 pg/mL - 10000 pg/mL; Sample type: Cell culture extracts, Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Cow, Human |
Target Information
| Target Symbol | NCDH |
|---|---|
| UniProt ID | P55289 |
| Function | Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. |
| Cellular Localization | Cell membrane. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
