Human Neprilysin ELISA Kit (RMEK-0152812)
Cat. No.: RMEK-0152812
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human Neprilysin ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Neprilysin protein in urine and cell culture supernatant. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Urine-5-8.4%; Inter-Assay-Urine-3-6.6% |
| Sensitivity | 97.2 pg/mL |
| Range | 156 pg/mL - 10000 pg/mL |
| Sample Type | Cell culture supernatant, Urine |
| Recovery | Cell culture supernatant-100-93% - 110%; Urine-90-80% - 98% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 97.2 pg/mL; Range: 156 pg/mL - 10000 pg/mL; Sample type: Cell culture supernatant, Urine; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | MME |
|---|---|
| UniProt ID | P08473 |
| Biomarker of SCs/CSCs | Colon Cancer |
| Function | Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. |
| Cellular Localization | Cell membrane. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
