Human Neuron specific Enolase ELISA Kit (RMEK-0152817)
Cat. No.: RMEK-0152817
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human Neuron specific Enolase ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Neuron specific Enolase protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. The technology. Quantitate Human Neuron specific Enolase with 23 pg/mL sensitivity. _x000D_
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Overall-5-4.3%; Inter-Assay-Overall-3-11.4% |
| Sensitivity | 23 pg/mL |
| Range | 312.5 pg/mL - 20000 pg/mL |
| Sample Type | Cell culture extracts, Cell culture supernatant, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts |
| Recovery | Serum-106-103% - 108%; Cell culture extracts-90-89% - 91%; Tissue Extracts-110-106% - 113%; Cell culture media-103-102% - 105%; Hep Plasma-109-104% - 114%; EDTA Plasma-105-96% - 118%; Cit plasma-102-100% - 105%; Cerebral Spinal Fluid-107-103% - 110% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 23 pg/mL; Range: 312.5 pg/mL - 20000 pg/mL; Sample type: Cell culture extracts, Cell culture supernatant, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | ENO2 |
|---|---|
| UniProt ID | P09104 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. |
| Cellular Localization | Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. |
| Development Stage | During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
