Human Nodal ELISA Kit (RMEK-0152818)
Cat. No.: RMEK-0152818
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human Nodal ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Nodal protein in cell culture media and cell culture supernatant. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Supernatant-8-8.4%; Inter-Assay-Supernatant-3-8.9% |
| Sensitivity | 0.32 ng/mL |
| Range | 0.781 ng/mL - 50 ng/mL |
| Sample Type | Cell culture media, Cell culture supernatant |
| Recovery | Cell culture supernatant-88-86% - 91%; Cell culture media-97-95% - 99% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 0.32 ng/mL; Range: 0.781 ng/mL - 50 ng/mL; Sample type: Cell culture media, Cell culture supernatant; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | NODAL |
|---|---|
| UniProt ID | Q96S42 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Essential for mesoderm formation and axial patterning during embryonic development. |
| Cellular Localization | Secreted. |
| Involvement in Disease | Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5). A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
