Human Osteopontin ELISA Kit (RMEK-0152827)
Cat. No.: RMEK-0152827
Category: ELISA Kits
INQUIRY
1 x 96 tests
Osteopontin Human ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Osteopontin in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Osteopontin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteopontin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Osteopontin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteopontin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This assay employs an antibody specific for Human Osteopontin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteopontin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Osteopontin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteopontin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | Multiple steps standard assay |
| Assay Type | Sandwich (quantitative) |
| Sensitivity | Sensitivity: 50 pg/mL |
| Range | 74.07 pg/mL - 18000 pg/mL |
| Sample Type | Cell culture supernatant, Milk, Plasma, Serum, Urine |
| Recovery | Cell culture supernatant-103.5-95% - 118%; Serum-89.92-88% - 108%; Plasma-92.41-90% - 109% |
| Key Features | Sensitivity: 50 pg/mL; Range: 74.07 pg/mL - 18000 pg/mL; Sample type: Cell culture supernatant, Milk, Plasma, Serum, Urine; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | SPP1 |
|---|---|
| UniProt ID | P10451 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. |
| Cellular Localization | Secreted. |
| Post-transcriptional Modifications | Extensively phosphorylated on clustered serine residues.N- and O-glycosylated.Phosphorylation sites are present in the extracelllular medium. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
