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Human Pancreas Cells (RMCL-00260196)

Cat. No.: RMCL-00260196

Category: Cell model; Organoid

INQUIRY 1 vial
A patient-derived next-generation organoid cancer model. This cell line was isolated from primary pancreatic cancer tissue. This tumor-derived model can be used in basic research and pharmacological screening applications.

Product Features

BSL BSL-1
Product Format Frozen
Applications 3D cell culture; Cancer research
Specific Applications Basic research, compound screening.
Product Type Human cells
Organism Homo sapiens, human
Tissue Pancreas
Morphology Organoid
Disease Cancer; Primary

QC

Bacterial & Fungal Testing Not detected
Mycoplasma Contamination Not detected
Virus Testing Cytomegalovirus (CMV): Not detected
Epstein-Barr virus (EBV): Not detected
Hepatitis B virus (HBV): Not detected
Human Immunodeficiency virus (HIV): Not detected
Human papillomavirus (HPV): Not detected

Storage & Shipping

Storage Conditions Vapor phase of liquid nitrogen
Reagents for Cryopreservation We recommend cryopreserving this model in Stem Cell Freezing Media.
Growth Properties Embedded 3D culture
Temperature 37°C
Atmosphere 95% Air, 5% CO2
Handling procedure Seeding density:  We recommend seeding this model at 0. 25 - 1 x 106/ viable cells in 100 µL of ECM per well of a 6-well plate.  ECM:  We recommend culturing this model in Cell Basement Membrane or Corning Matrigel. Include 10 µM ROCK Inhibitor Y-27632 in medium for the first 2-3 days following subculture.  For a brief overview of the thawing procedure see our quickstart guide Thawing Cryopreserved Human Organoids.

For research use only. Not for clinical use.