Human PCSK9 ELISA Kit (RMEK-0152835)
Cat. No.: RMEK-0152835
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human PCSK9 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PCSK9 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Human Serum-5-4.4%; Inter-Assay-Human Serum-3-4.6% |
| Sensitivity | 68 pg/mL |
| Range | 0.5 ng/mL - 32 ng/mL |
| Sample Type | Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts |
| Recovery | Cell culture supernatant-106-103% - 109%; Serum-105-104% - 107%; Cell culture extracts-92-91% - 94%; Hep Plasma-111-107% - 115%; EDTA Plasma-96-93% - 100%; Cit plasma-104-102% - 106% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 68 pg/mL; Range: 0.5 ng/mL - 32 ng/mL; Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | PCSK9 |
|---|---|
| UniProt ID | Q8NBP7 |
| Biomarker of SCs/CSCs | Triple Negative Breast Cancer |
| Function | May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. |
| Cellular Localization | Secreted. |
| Post-transcriptional Modifications | The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme. |
| Involvement in Disease | Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3). FH3 inheritance is autosomal dominant. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
