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Human Rectosigmoid junction Cells

Human Rectosigmoid junction Cells (RMCL-00260098)

Cat. No.: RMCL-00260098

Category: Cell model; Organoid

INQUIRY 1 vial
A patient-derived next-generation cancer model. This cell line was isolated from primary adenocarcinoma of large intestine tissue. This tumor-derived model can be used in basic research and pharmacological screening applications.

Product Features

BSL BSL-1
Product Format Frozen
Applications 3D cell culture; Cancer research
Specific Applications Basic research, compound screening.
Product Type Human cells
Organism Homo sapiens, human
Tissue Large intestine; Rectosigmoid junction
Morphology Organoid
Disease Adenocarcinoma; Primary

QC

Bacterial & Fungal Testing Not detected
Mycoplasma Contamination Not detected
Virus Testing Cytomegalovirus (CMV): Not detected
Human papillomavirus (HPV): Not detected
Human Immunodeficiency virus (HIV): Not detected
Epstein-Barr virus (EBV): Not detected
Hepatitis B virus (HBV): Not detected

Doner Information

Gender Male

Storage & Shipping

Storage Conditions Vapor phase of liquid nitrogen
Reagents for Cryopreservation We recommend cryopreserving this model in Stem Cell Freezing Media.
Unpacking and Storage Instructions Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
Growth Properties Embedded 3D culture
Temperature 37°C
Atmosphere 95% Air, 5% CO2
Handling procedure We recommend cryopreserving this model in Stem Cell Freezing Media. For a brief overview of the cryopreservation procedure for organoids see our quickstart guide Organoid Cryopreservation Protocol. For more details on the handling and culture of organoids see our methods paper in Current Protocols in Cell Biology.

For research use only. Not for clinical use.