Human SCF ELISA Kit (RMEK-0152875)
Cat. No.: RMEK-0152875
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human Stem Cell Factor (SCF) ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SCF protein in human plasma, serum and cell culture supernatant samples. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Pooled Serum-24-6%; Inter-Assay-Pooled Serum-6-2% |
| Sensitivity | 0.51 pg/mL |
| Range | 15.63 pg/mL - 1000 pg/mL |
| Sample Type | Cell culture media, Cit plasma, Hep Plasma, Plasma, Serum |
| Recovery | Serum-102-92% - 111%; Cell culture media-106-100% - 113%; Hep Plasma-105-91% - 115%; Cit plasma-89-79% - 103% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 0.51 pg/mL; Range: 15.63 pg/mL - 1000 pg/mL; Sample type: Cell culture media, Cit plasma, Hep Plasma, Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | SCF |
|---|---|
| UniProt ID | P21583 |
| Function | Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. |
| Cellular Localization | Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. |
| Development Stage | Acts in the early stages of hematopoiesis. |
| Post-transcriptional Modifications | A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.A soluble form exists as a cleavage product of the extracellular domain. |
| Involvement in Disease | Hyperpigmentation with or without hypopigmentation, familial progressiveDeafness, congenital, unilateral or asymmetric |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
