Human SFN ELISA Kit (RMEK-0152898)
Cat. No.: RMEK-0152898
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human SFN ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SFN protein in edta plasma, saliva, serum, tissue extracts, and cell culture extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Saliva-6-7.4%; Inter-Assay-Saliva-3-9.9% |
| Sensitivity | 115 pg/mL |
| Range | 0.156 ng/mL - 10 ng/mL |
| Sample Type | Cell culture extracts, EDTA Plasma, Saliva, Serum, Tissue Extracts |
| Recovery | Saliva-99-89% - 108%; Serum-92-90% - 96%; Cell culture extracts-91-76% - 100%; Tissue Extracts-98-96% - 99%; EDTA Plasma-84-82% - 89% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 115 pg/mL; Range: 0.156 ng/mL - 10 ng/mL; Sample type: Cell culture extracts, EDTA Plasma, Saliva, Serum, Tissue Extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | SFN |
|---|---|
| UniProt ID | P31947 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.p53-regulated inhibitor of G2/M progression. |
| Cellular Localization | Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway. |
| Post-transcriptional Modifications | Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
