Human Syndecan-1 ELISA Kit(CD138) (RMEK-0152939)
Cat. No.: RMEK-0152939
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human Syndecan-1 ELISA (CD138) kit is designed for the quantitative measurement of Syndecan-1 in Human serum, plasma, buffered solutions and supernatants. A monoclonal antibody specific for Syndecan-1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Syndecan-1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Syndecan-1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Syndecan-1 present in the samples. This kit will recognize both endogenous and recombinant Human Syndecan-1.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | Multiple steps standard assay |
| Assay Time | 1.75 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Overall-6-6.2%; Inter-Assay-Overall-6-10.2% |
| Sensitivity | Sensitivity: 4.94 ng/mL |
| Range | 8 ng/mL - 256 ng/mL |
| Sample Type | Cell culture supernatant, Plasma, Serum |
| Key Features | Sensitivity: 4.94 ng/mL; Range: 8 ng/mL - 256 ng/mL; Sample type: Cell culture supernatant, Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | Syndecan1 |
|---|---|
| UniProt ID | P18827 |
| Function | Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. |
| Cellular Localization | Membrane. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
