Human Thrombomodulin ELISA Kit (RMEK-0152959)
Cat. No.: RMEK-0152959
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human Thrombomodulin ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Thrombomodulin protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, urine, and tissue extracts. The technology. Quantitate Human Thrombomodulin with 3 pg/mL sensitivity. _x000D_
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Human Serum-8-3.1%; Inter-Assay-Human Serum-3-9.4% |
| Sensitivity | 3 pg/mL |
| Range | 20.3 pg/mL - 1300 pg/mL |
| Sample Type | Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Urine |
| Recovery | Cell culture supernatant-90-88% - 91%; Urine-106-102% - 112%; Serum-97-96% - 99%; Cell culture extracts-94-92% - 96%; Tissue Extracts-105-101% - 110%; Hep Plasma-99-98% - 100%; EDTA Plasma-96-94% - 98%; Cit plasma-96-92% - 98% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 3 pg/mL; Range: 20.3 pg/mL - 1300 pg/mL; Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Urine; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | THBD |
|---|---|
| UniProt ID | P07204 |
| Biomarker of SCs/CSCs | Luminal A Breast Cancer |
| Function | Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. |
| Cellular Localization | Membrane. |
| Post-transcriptional Modifications | N-glycosylated.The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. |
| Involvement in Disease | Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD). A hemostatic disorder characterized by a tendency to thrombosis. Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
