Human Thrombomodulin ELISA Kit(CD141) (RMEK-0152960)
Cat. No.: RMEK-0152960
Category: ELISA Kits
INQUIRY
1 x 96 tests
Thrombomodulin (CD141) Human in vitro ELISA kit is designed for the quantitative measurement of Thrombomodulin (CD141) in serum, plasma, buffered solutions and supernatants. A monoclonal antibody specific for Thrombomodulin has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Thrombomodulin concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Thrombomodulin are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Thrombomodulin present in the samples. This kit will recognize both endogenous and recombinant Human Thrombomodulin.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | Multiple steps standard assay |
| Assay Time | 1.75 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-6-3.9%; Inter-Assay-Serum-6-9.8% |
| Sensitivity | Sensitivity: 0.31 ng/mL |
| Range | 0.625 ng/mL - 20 ng/mL |
| Sample Type | Cell culture supernatant, Plasma, Serum |
| Key Features | Sensitivity: 0.31 ng/mL; Range: 0.625 ng/mL - 20 ng/mL; Sample type: Cell culture supernatant, Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | THBD |
|---|---|
| UniProt ID | P07204 |
| Biomarker of SCs/CSCs | Luminal A Breast Cancer |
| Function | Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. |
| Cellular Localization | Membrane. |
| Post-transcriptional Modifications | N-glycosylated.The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. |
| Involvement in Disease | Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD). A hemostatic disorder characterized by a tendency to thrombosis. Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
