Human Vimentin Profiling ELISA Kit (RMEK-0153012)
Cat. No.: RMEK-0153012
Category: ELISA Kits
INQUIRY
1 x 96 tests
Vimentin Human Profiling in vitro ELISA kit is designed for the accurate qualitative measurement of total vimentin protein in Human samples. The assay employs an antibody specific to Vimentin coated onto well plate strips. Samples are pipetted into the wells and Vimentin present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-Vimentin detector antibody is added. After washing away unbound detector antibody, HRP-conjugated label specific for the detector antibody is pipetted into the wells. The wells are again washed, a TMB development solution is added to the wells and blue color develops in proportion to the amount of bound Vimentin.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | Multiple steps standard assay |
| Assay Type | Quantitative |
| Precision | Intra-Assay-Overall-3-7.5%; Inter-Assay-Overall-3-12% |
| Sensitivity | Sensitivity: 8 µg/mL |
| Sample Type | Adherent cells, Suspension cells, Tissue Homogenate, Tissue Lysate |
| Recovery | Cell culture media-80-71% - 87%; Fetal Bovine Serum-89-81% - 93%; Bovine Serum Albumin-89-83% - 84% |
| Key Features | Sensitivity: 8 µg/mL; Sample type: Adherent cells, Suspension cells, Tissue Homogenate, Tissue Lysate; Detection method: Colorimetric; Assay type: Quantitative; Reacts with: Human |
Target Information
| Target Symbol | VIM |
|---|---|
| UniProt ID | P08670 |
| Biomarker of SCs/CSCs | Skin Cancer |
| Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. |
| Cellular Localization | Cytoplasm. |
| Domain | The central alpha-helical coiled-coil rod region mediates elementary homodimerization.The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. |
| Post-transcriptional Modifications | Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. |
| Involvement in Disease | Cataract 30 |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
