Human WISP-1 ELISA Kit(CCN4) (RMEK-0153026)
Cat. No.: RMEK-0153026
Category: ELISA Kits
INQUIRY
1 x 96 tests
WISP-1 (CCN4) Human ELISA kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human WISP-1 in serum, plasma (collect plasma using EDTA as an anticoagulant. Heparin and Citrate are not recommended as anticoagulants for use in this assay) and cell culture supernatants. This assay employs an antibody specific for Human WISP-1coated on a 96-well plate. Standards and samples are pipetted into the wells and WISP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human WISP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of WISP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | Multiple steps standard assay |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Overall--< 10%; Inter-Assay-Overall--< 12% |
| Sensitivity | Sensitivity: 50 pg/mL |
| Range | 0.061 ng/mL - 15 ng/mL |
| Sample Type | Cell culture supernatant, Plasma, Serum |
| Recovery | Cell culture supernatant-93.12-87% - 101%; Serum-90.42-80% - 97%; Plasma-127.1-119% - 135% |
| Key Features | Sensitivity: 50 pg/mL; Range: 0.061 ng/mL - 15 ng/mL; Sample type: Cell culture supernatant, Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | WISP1 |
|---|---|
| UniProt ID | O95388 |
| Function | Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. |
| Cellular Localization | Secreted |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
