Human YB1 ELISA Kit(YBX1) (RMEK-0153029)
Cat. No.: RMEK-0153029
Category: ELISA Kits
INQUIRY
1 x 96 tests
Human YB1 ELISA Kit (YBX1) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of YB1 (YBX1) protein in cell lysate. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Extract-8-3.9%; Inter-Assay-Extract-3-4.9% |
| Sensitivity | 0.85 ng/mL |
| Range | 0.9 ng/mL - 60 ng/mL |
| Sample Type | Cell Lysate |
| Recovery | Cell Lysate-114-106% - 120% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 0.85 ng/mL; Range: 0.9 ng/mL - 60 ng/mL; Sample type: Cell Lysate; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Human |
Target Information
| Target Symbol | YB1 |
|---|---|
| UniProt ID | P67809 |
| Function | Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability.The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation. |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasmic granule. Secreted. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between nucleus and cytoplasm. Predominantly cytoplasmic in proliferating cells. Cytotoxic stress and DNA damage enhance translocation to the nucleus. Localized with DDX1, MBNL1 and TIAL1 in stress granules upon stress. Secreted by mesangial and monocytic cells after inflammatory challenges. Translocates from the cytoplasm to the nucleus after and colocalizes with APEX1 in nuclear speckles after genotoxic stress. |
| Post-transcriptional Modifications | Ubiquitinated by RBBP6; leading to a decrease of YBX1 transcativational ability.In the absence of phosphorylation the protein is retained in the cytoplasm.Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
