Human/Mouse Oct4 Antibody Pair - BSA and Azide free (RMAB-0252024)
Cat. No.: RMAB-0252024
Category: Antibody Pair
INQUIRY
10 x 96 tests
The Antibody Pair can be used to quantify Human/Mouse Oct4. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/mL as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results._x000D_
Product Features
| Conjugate | Unconjugated capture and detector antibodies |
|---|---|
| Species Reactivity | Mouse, Human |
| Range | 1.76 ng/mL - 112.5 ng/mL |
| Applications | Sandwich ELISA |
| Key Features | Unconjugated capture and detector antibodies; Adaptable to any antibody pair-based assay format; Antibody concentration ~ 1 mg/mL; BSA and azide free buffer - ready for conjugation; Reacts with: Mouse, Human |
Target Information
| Target Symbol | POU5F1 |
|---|---|
| Target Name | POU domain, class 5, transcription factor 1 |
| UniProt ID | Q01860 |
| Cellular Localization | Nucleus. Expressed in a diffuse and slightly punctuate pattern. |
| Developmental Stage | Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation. |
| Function | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
| Post-translational Modifications | Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression. Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. |
| Domain | The POU-specific domain mediates interaction with PKM2. |
| Sequence Similarities | Belongs to the POU transcription factor family. Class-5 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. |
Storage & Shipping
| Storage & Shipping | Store at 4°C. Please refer to protocols. |
|---|
For research use only. Not for clinical use.
