Human/Mouse Oct4 ELISA Kit (RMEK-0153034)
Cat. No.: RMEK-0153034
Category: ELISA Kits
INQUIRY
1 x 96 tests
Oct4 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Oct4 protein in cell culture extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse, Human |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Cell extract-8-9.8% |
| Sensitivity | 596 pg/mL |
| Range | 1.76 ng/mL - 112.5 ng/mL |
| Sample Type | Cell culture extracts |
| Recovery | Cell culture extracts-102% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 596 pg/mL; Range: 1.76 ng/mL - 112.5 ng/mL; Sample type: Cell culture extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse, Human |
Target Information
| Target Symbol | Human/Oct4 |
|---|---|
| UniProt ID | Q01860 |
| Function | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
| Cellular Localization | Nucleus. Expressed in a diffuse and slightly punctuate pattern. |
| Development Stage | Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation. |
| Domain | The POU-specific domain mediates interaction with PKM2. |
| Post-transcriptional Modifications | Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
