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Intestinal Organoid Kit (RMMS-00560162)

Cat. No.: RMMS-00560162

Category: Assay Kits

Research Area: Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology

INQUIRY 1 Kit
Serum-free medium kit for establishment and maintenance of human intestinal organoids from hPSCs

Product Features

Cell Type Endoderm, PSC-Derived, Intestinal Cells
Formulation Serum-Free
Species Human
Applications Cell Culture, Differentiation, Organoid Culture
Description This product is a serum-free cell culture medium system that enables robust and efficient generation of human pluripotent stem cell (hPSC)-derived intestinal organoids in a simple, three-stage protocol. Using this kit, hPSCs are directed through induction of definitive endoderm, mid-/hindgut spheroids, and generation of intestinal organoids that can be maintained long-term through passaging or cryopreserved. The organoids exhibit a cellular composition and organization that models the developing intestinal epithelium and associated mesenchyme, making them a convenient model system with direct relevance to the developing intestine. Intestinal Organoid Kit is and has been optimized to increase efficiency and reproducibility of organoid formation and expansion across hPSC lines. Human intestinal organoids can be used as a model system for studying intestinal development and cell biology, intestinal inflammation, intestinal regeneration, microbial interaction, disease modeling, drug discovery, and compound screening. The kit is optimized for differentiation of cells maintained in. For extended culture and passaging, the kit components required for organoid maintenance can be purchased as Intestinal Organoid Growth Medium.

Storage & Shipping

Storage -20°C or colder
Shipping Shipped on gel packs.

For research use only. Not for clinical use.