iPSC-derived CD34+ Cells, BXS0117 (RMCL-00260338)
Cat. No.: RMCL-00260338
Category: Primary cell
INQUIRY
1 vial
iPSC-derived CD34+ Cells, BXS0117 are induced pluripotent stem cells that have been differentiated into hematopoietic progentitor CD34+ cells. These cells can be further differentiated down common lymphoid progenitor and common myeloid progenitor lineages. iPSC-derived CD34+ Cells can be used in cancer immunology research, drug development, toxicity screening, and blood lineage differentiation studies.
Product Features
| BSL | BSL-2 |
|---|---|
| Product Format | Frozen |
| Applications | High-throughput screening; Immunology; Quality control; Stem cell research; Toxicology; Immuno-oncology; Cell and gene therapy (CGT) development |
| Specific Applications | Biopharma: Cancer immunology, drug development, toxicity screening, and blood lineage differentiation studies. |
| Product Type | Human cells |
| Organism | Homo sapiens, human |
| Morphology | Rounded |
| STR Profiling | Amelogenin: X CSF1PO: 10 ,12 D13S17: 9 ,12 D16S539: 11 D5S818: 12 D7S820: 9 ,13 TH01: 7 ,9 TPOX: 8 ,11 vWA: 19 |
QC
| Bacterial & Fungal Testing | Not detected |
|---|---|
| Mycoplasma Contamination | Not detected |
| Virus Testing | Hepatitis B virus (HBV): Not detected Cytomegalovirus (CMV): Not detected Human Immunodeficiency virus (HIV): Not detected Epstein-Barr virus (EBV): Not detected Human papillomavirus (HPV): Not detected |
Doner Information
| Gender | Female |
|---|---|
| Age | 27 years |
| Ethnicity | Asian |
Storage & Shipping
| Storage Conditions | Vapor phase of liquid nitrogen |
|---|---|
| Unpacking and Storage Instructions | Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Growth Properties | Suspension |
| Temperature | 37°C |
| Atmosphere | 95% Air, 5% CO2 |
| Handling procedure | Refer to the batch specific information for the total number of viable cells recovered from this lot of this product Lot is ~ 2. 8 x 106 Using the total number of viable cells, customers have to decide seeding for their experiments and applications. Prepare the desired combinations of culture dishes with required media. Place dishes in a 37°C, 5% CO2, humidified incubator and allow the media to pre-equilibrate to temperature and pH for 30 minutes prior to adding cells. While the culture dishes equilibrate, remove one vial of this product from storage and thaw the cells by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 1 to 2 minutes). Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All operations from this point onward should be carried out under strict aseptic conditions. Add 4ml of base medium or complete growth media – into a sterile conical tube. Using a sterile pipette, transfer cells from the cryovial to the conical tube. Centrifuge at 200-300xg for 5min, remove supernatant and re suspend the pellet in complete growth medium Transfer cell suspension to each of the pre-equilibrated culture dishes in the required seeding density, gently rock each dishes to evenly distribute the cells. Place the seeded culture flasks in the incubator at 37°C with a 5% CO2 atmosphere. Incubate for at least 24 hours before processing the cells further. |
For research use only. Not for clinical use.
