iPSC-derived Monocytes (RMCL-00260218)
Cat. No.: RMCL-00260218
Category: Primary cell
INQUIRY
1 vial
iPSC-derived Monocytes are induced pluripotent stem cells that have been differentiated into CD14+ monocytes. These cells can be used in cancer immunology research, drug development, and toxicity screening.
Product Features
| BSL | BSL-2 |
|---|---|
| Product Format | Frozen |
| Applications | High-throughput screening; Immunology; Stem cell research; Toxicology; Immuno-oncology; Cell and gene therapy (CGT) development |
| Specific Applications | Biopharma: Cancer immunology, drug development, and toxicity screening |
| Functional Test | Phagocytosis assay; macrophage and dendritic cell differentiation |
| Product Type | Human cells |
| Organism | Homo sapiens, human |
| Morphology | Rounded |
| Disease | Normal |
| STR Profiling | Amelogenin: X,Y CSF1PO: 7,11 D13S17: 11,13 D16S539: 12 D5S818: 11 D7S820: 9,10 TPOX: 8,11 TH01: 9.3 vWA: 14,19 |
QC
| Bacterial & Fungal Testing | Not detected |
|---|---|
| Mycoplasma Contamination | Not detected |
| Virus Testing | Hepatitis B virus (HBV): Not detected Hepatitis C virus (HCV): Not detected Human immunodeficiency virus 1 (HIV-1): Not detected Human immunodeficiency virus 2 (HIV-2): Not detected |
Doner Information
| Gender | Male |
|---|---|
| Age | Neonate |
| Ethnicity | Unknown |
Storage & Shipping
| Storage Conditions | Vapor phase of liquid nitrogen |
|---|---|
| Unpacking and Storage Instructions | Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Growth Properties | Suspension |
| Temperature | 37°C |
| Atmosphere | 95% Air, 5% CO2 |
| Handling procedure | Refer to the batch specific information for the total number of viable cells. Using the total number of viable cells, customers have to decide seeding for their experiments and applications. Prepare the desired combinations of culture dishes with application specific media. Place dishes in a 37°C, 5% CO2, humidified incubator and allow the media to pre-equilibrate to temperature and pH for 30 minutes prior to adding cells. While the culture dishes equilibrate, remove one vial of iPSC-derived Monocytes from storage and thaw the cells in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 1 to 2 minutes). Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All operations from this point onward should be carried out under strict aseptic conditions. Add 4 ml of RPMI 1640 with or without 10% FBS or complete growth media for specific applications – into a sterile conical tube. Using a sterile pipette, transfer cells from the cryovial to the conical tube. Centrifuge at 200-300 x g for 5 min, remove supernatant and re suspend the pellet in required medium and take an aliquot for cell counting using Vi-cell Transfer cell suspension to each of the pre-equilibrated culture dishes in the required seeding density depending the application and gently rock each dishes to evenly distribute the cells. Place the seeded culture flasks in the incubator at 37°C with a 5% CO2 atmosphere. Incubate for at least 24 hours before processing the cells further. |
For research use only. Not for clinical use.
