MEF (DR4) (RMCL-00260308)
Cat. No.: RMCL-00260308
Category: Cell Lines
INQUIRY
1 vial
MEF (DR4) is a fibroblast cell that was established from embryonic day 14 (E14) DR4 mouse embryos obtained from The Jackson Laboratory. The cells can be used as a feeder layer to support the growth of engineered embryonic stem (ES) cells with multiple drug selections and for the maintenance of ES cells in the undifferentiated state.
Product Features
| BSL | BSL-1 |
|---|---|
| Product Format | Frozen |
| Applications | Stem cell research |
| Specific Applications | Can be used to produce feeder cells with multiple drug resistance. Tis product can be used as a feeder layer to support the growth of engineered embryonic stem (ES) cells with multiple drug selections and for the maintenance of ES cells in the undifferentiated state. |
| Product Type | Animal cells |
| Strain | DR4 |
| Organism | Mus musculus, mouse |
| Tissue | Embryo |
| Morphology | fibroblast |
| Cell Type | Fibroblast |
QC
| Mycoplasma Contamination | Not detected |
|---|
Doner Information
| Gender | Male and female mixed |
|---|---|
| Age | 14 days gestation |
Storage & Shipping
| Storage Conditions | Vapor phase of liquid nitrogen |
|---|---|
| Reagents for Cryopreservation | Complete growth medium supplemented with 40% (v/v) FBS and 10% (v/v) DMSO |
| Unpacking and Storage Instructions | Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Growth Properties | Adherent |
| Temperature | 37°C |
| Atmosphere | 95% Air, 5% CO2 |
| Handling procedure | It is not necessary to coat flasks with gelatin prior to plating cells, if tissue culture quality flasks are used. To insure the highest level of viability, be sure to warm media to 37°C before using it on the cells. It is recommended to seed cells at 1. 2 X 104 cells/cm2 post-thaw. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Remove the vial from the water bath as soon as the contents are half way thawed (approximately 90 seconds), and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions. Transfer the vial’s contents plus 5 mL of complete growth medium to a 15 mL centrifuge tube. Use an additional 1 mL of media to rinse the vial and transfer the liquid to the 15 mL tube. Add 4 mL of complete DMEM to bring the total volume to 10 mL. Gently mix and pellet the cells by centrifugation at 270 x g for 5 minutes. Discard the supernatant and resuspend the cells with 10mL fresh complete growth medium (warm) and count cells. If necessary, add more fresh complete growth medium (warm) to obtain a seeding density of 1. 2 X 104cells/cm2. Transfer appropriate volumes of cell suspension to culture vessels. Add more complete growth medium to obtain the total volume recommended for the culture vessels seeded. Incubate 37°C in a 5% CO2 in air atmosphere. Fluid change twice a week or when pH decreases. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7. 0 to 7. 6). |
For research use only. Not for clinical use.
