Midbrain Organoid Differentiation Kit (RMMS-00560119)
Cat. No.: RMMS-00560119
Category: Cell Differentiation Kits
Research Area: Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Organoids
INQUIRY
1 Kit
Cell culture medium kit for robust generation of midbrain neural organoids from human pluripotent stem cells.
Product Features
| Cell Type | Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Pluripotent Stem Cells |
|---|---|
| Formulation | Serum-Free |
| Applications | Cell Culture, Characterization, Differentiation, Functional Assay, Immunofluorescence, Organoid Culture, Phenotyping, Spheroid Culture |
| Description | Robustly generate three-dimensional, patterned brain organoid cultures from human pluripotent stem cells without matrix embedding by using Midbrain Organoid Differentiation Kit. When paired with , this serum-free cell culture media can prevent organoid fusion and support the generation of over 500 organoids per kit for higher-powered statistical replicates and more detailed longitudinal study. Adapted from protocols by Dr. Sergiu Paşca, the brain-region-specific organoids generated with this kit are three-dimensional in vitro models with a cellular composition and structural organization representative of the developing human midbrain. They are consistently sized with reproducible morphology between various cell lines, and provide a reliable model for the study of dopaminergic neurons within a functional 3D system. Midbrain organoids can be combined with organoids generated using the Dorsal Forebrain Organoid Differentiation Kit to generate cortico-striatal cultures. Organoids can be maintained with Neural Organoid Maintenance Kit to support long-term culture survival (> 50 days) for predictive assays, high-throughput phenotypic screening, and neurotoxicity assays. |
Storage & Shipping
| Storage | -20°C or colder |
|---|---|
| Shipping | Shipped on gel packs. |
For research use only. Not for clinical use.
