MITC-STO (RMCL-00260296)
Cat. No.: RMCL-00260296
Category: Cell Lines
INQUIRY
1 vial
MITC-STO mitomycin C-treated feeder cells are fibroblasts derived from a mouse embryo. These cells can be used as feeder cells to support the growth of other cells. This cell line has applications in stem cell research.
Product Features
| BSL | BSL-1 |
|---|---|
| Product Format | Frozen |
| Applications | Stem cell research |
| Specific Applications | Mitomycin C-treated cells are used as a feeder layer |
| Product Type | Animal cells |
| Organism | Mus musculus, mouse |
| Tissue | Embryo |
| Morphology | fibroblast |
| Cell Type | Fibroblast |
QC
| Mycoplasma Contamination | Not detected |
|---|
Doner Information
| Age | Embryo |
|---|
Storage & Shipping
| Storage Conditions | Vapor phase of liquid nitrogen |
|---|---|
| Reagents for Cryopreservation | Complete growth medium supplemented with 5% (v/v) DMSO |
| Unpacking and Storage Instructions | Check all containers for leakage or breakage. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use. |
| Growth Properties | Adherent |
| Temperature | 37°C |
| Atmosphere | 95% Air, 5% CO2 |
| Handling procedure | Handling Procedure for Frozen CellsTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability. SAFETY PRECAUTION: highly recommends that protective gloves and clothing always be used and a full face mask always be worn when handling frozen vials. It is important to note that some vials leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vessel exploding or blowing off its cap with dangerous force creating flying debris. 1. Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes). 2. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions. Transfer the vial contents to a centrifuge tube containing 9. 0 ml complete culture medium and spin at approximately 125 x g for 5 to7 minutes. 3. Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio) and dispense into a culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7. 0 to 7. 6). 4. Incubate the culture at 37°C in a suitable incubator. |
For research use only. Not for clinical use.
