Monkey Cardiac Troponin I ELISA Kit- Extracellular (RMEK-0153134)
Cat. No.: RMEK-0153134
Category: ELISA Kits
INQUIRY
1 x 96 tests
Monkey Cardiac Troponin I kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cardiac Troponin I protein in Serum, Cit Plasma, EDTA Plasma, Cell culture media. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Monkey |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-5.6%; Inter-Assay-Serum-3-3.2% |
| Sensitivity | 2.075 ng/mL |
| Range | 2.656 ng/mL - 170 ng/mL |
| Sample Type | Cell culture media, Cit plasma, EDTA Plasma, Serum |
| Recovery | Serum-88-81% - 96%; Cell culture media-109-103% - 114%; EDTA Plasma-92-90% - 94%; Cit plasma-89-86% - 94% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 2.075 ng/mL; Range: 2.656 ng/mL - 170 ng/mL; Sample type: Cell culture media, Cit plasma, EDTA Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Monkey |
Target Information
| Target Symbol | TNNI3 |
|---|---|
| UniProt ID | P19429 |
| Function | Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. |
| Post-transcriptional Modifications | Phosphorylated at Ser-42 and Ser-44 by PRKCE; phosphorylation increases myocardium contractile dysfunction (By similarity). Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-31. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T). |
| Involvement in Disease | Cardiomyopathy, familial hypertrophic, 7 (CMH7): A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. The disease is caused by variants affecting the gene represented in this entry. Cardiomyopathy, familial restrictive 1 (RCM1): A heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. The disease is caused by variants affecting the gene represented in this entry. Cardiomyopathy, dilated, 2A (CMD2A): A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. The disease is caused by variants affecting the gene represented in this entry. Cardiomyopathy, dilated, 1FF (CMD1FF): A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. The disease is caused by variants affecting the gene represented in this entry. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
