Monocyte Kit (RMMS-00560117)
Cat. No.: RMMS-00560117
Category: Specialized Medium Kits
Research Area: Disease Modeling, Immunology, Stem Cell Biology
INQUIRY
1 Kit
For differentiation of human pluripotent stem cells (hPSCs) to monocytes
Product Features
| Cell Type | Dendritic Cells, Macrophages, Monocytes, Myeloid Cells, Pluripotent Stem Cells |
|---|---|
| Formulation | Serum-Free |
| Species | Human |
| Applications | Cell Culture, Differentiation, Expansion |
| Description | Differentiate human pluripotent stem cells (hPSCs) to monocytes expressing CD14 with the feeder-free and serum-free Monocyte Kit. The simple protocol is performed in 2D adherent cultures. During the first 3 days, Medium A induces cells toward mesoderm. For the subsequent 4 days, mesodermal cells are further differentiated toward the hematopoietic lineage using Medium B. At Day 7, the medium is changed to Monocyte Differentiation Medium, which facilitates the differentiation to monocytes. CD14+ monocytes can be harvested directly from the culture supernatant starting as early as Day 14 and can be repeatedly harvested during the rest of the culture period. Peak CD14+ frequency is typically 60 - 80%. The hPSC-derived monocytes can be further differentiated to dendritic cells or macrophages using the Dendritic Cell Culture Kit or the-SF Macrophage Medium, respectively. For your convenience, components required for the preparation of the Monocyte Differentiation Medium, SFEM II and Monocyte Differentiation Supplement (100X), are also available for purchase individually. |
Storage & Shipping
| Storage | -20°C or colder |
|---|---|
| Shipping | Shipped on gel packs. |
For research use only. Not for clinical use.
