Mouse CD137 ELISA Kit (RMEK-0153622)
Cat. No.: RMEK-0153622
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse CD137 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD137 protein in cell culture extracts, cell culture supernatant, plasma, serum, and tissue extracts. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Supernatant-5-3.9%; Inter-Assay-Supernatant-3-4.8% |
| Sensitivity | 14 pg/mL |
| Range | 78.125 pg/mL - 5000 pg/mL |
| Sample Type | Cell culture extracts, Cell culture supernatant, Plasma, Serum, Tissue Extracts |
| Recovery | Serum-108.8-99.9% - 114.3%; Cell culture media-94.5-88.4% - 103.7%; EDTA Plasma-106.3-103.5% - 110.1%; Cit plasma-120.5-116.6% - 124.1% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 14 pg/mL; Range: 78.125 pg/mL - 5000 pg/mL; Sample type: Cell culture extracts, Cell culture supernatant, Plasma, Serum, Tissue Extracts; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | CD137 |
|---|---|
| UniProt ID | Q07011 |
| Function | Receptor for TNFSF14/4-1BBL. Possibly active during T cell activation. |
| Cellular Localization | Membrane. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.