Mouse CD27 ELISA Kit (RMEK-0153627)
Cat. No.: RMEK-0153627
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse CD27 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD27 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-5-5.3%; Inter-Assay-Serum-3-2% |
| Sensitivity | 10.9 pg/mL |
| Range | 31.3 pg/mL - 2000 pg/mL |
| Sample Type | Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Cell culture supernatant-104-98% - 108%; Serum-100-93% - 106%; Hep Plasma-105-99% - 109%; EDTA Plasma-90-79% - 99%; Cit plasma-94-88% - 98% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 10.9 pg/mL; Range: 31.3 pg/mL - 2000 pg/mL; Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | CD27 |
|---|---|
| UniProt ID | P26842 |
| Function | Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. |
| Cellular Localization | Membrane. |
| Post-transcriptional Modifications | Phosphorylated and O-glycosylated. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
