Mouse CD40L ELISA Kit(TNFSF5) (RMEK-0153630)
Cat. No.: RMEK-0153630
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse CD40L ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse CD40L protein in serum, plasma, and cell culture samples. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-4.6%; Inter-Assay-Serum-3-3.4% |
| Sensitivity | 11.77 pg/mL |
| Range | 17.19 pg/mL - 1100 pg/mL |
| Sample Type | Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Serum-102-98% - 108%; Cell culture media-98-89% - 103%; Hep Plasma-97-91% - 100%; EDTA Plasma-106-105% - 108%; Cit plasma-103-100% - 106% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 11.77 pg/mL; Range: 17.19 pg/mL - 1100 pg/mL; Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | CD40L |
|---|---|
| UniProt ID | P29965 |
| Function | Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. |
| Cellular Localization | Secreted and Cell membrane. |
| Post-transcriptional Modifications | The soluble form derives from the membrane form by proteolytic processing.N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.Not O-glycosylated. |
| Involvement in Disease | Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
