Mouse CD86 ELISA Kit (RMEK-0153634)
Cat. No.: RMEK-0153634
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse CD86 kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD86 protein in Serum, Hep Plasma, Cit Plasma, EDTA Plasma. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-8.9%; Inter-Assay-Serum-3-4.74% |
| Sensitivity | 2.137 pg/mL |
| Range | 7.813 pg/mL - 500 pg/mL |
| Sample Type | Cit plasma, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Serum-113-104% - 120%; Hep Plasma-105-99% - 112%; EDTA Plasma-103-96% - 112%; Cit plasma-103-100% - 112% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 2.137 pg/mL; Range: 7.813 pg/mL - 500 pg/mL; Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | CD86 |
|---|---|
| UniProt ID | P42081 |
| Function | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. |
| Cellular Localization | Membrane. |
| Post-transcriptional Modifications | Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
