Mouse CYR61 ELISA Kit (RMEK-0153681)
Cat. No.: RMEK-0153681
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse CYR61 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CYR61 protein in cell culture media, cell culture supernatant, edta plasma, and serum. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-3%; Inter-Assay-Serum-5-3.1% |
| Sensitivity | 10.1 pg/mL |
| Range | 62.5 pg/mL - 4000 pg/mL |
| Sample Type | Cell culture media, Cell culture supernatant, EDTA Plasma, Serum |
| Recovery | Cell culture supernatant-92-89% - 94%; Serum-112-108% - 115%; Cell culture media-88-83% - 95%; EDTA Plasma-127-125% - 133% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 10.1 pg/mL; Range: 62.5 pg/mL - 4000 pg/mL; Sample type: Cell culture media, Cell culture supernatant, EDTA Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | CYR61 |
|---|---|
| UniProt ID | O00622 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. |
| Cellular Localization | Secreted. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
