Mouse DKK1 ELISA Kit (RMEK-0153698)
Cat. No.: RMEK-0153698
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse DKK1 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DKK1 protein in cell culture supernatant, cit plasma, and serum. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Overall-8-5.2%; Inter-Assay-Overall-3-5.26% |
| Sensitivity | 6.66 pg/mL |
| Range | 39.1 pg/mL - 2500 pg/mL |
| Sample Type | Cell culture supernatant, Cit plasma, Serum |
| Recovery | Serum-114-109% - 120%; Cell culture media-99-93% - 105%; Cit plasma-112-99% - 121% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 6.66 pg/mL; Range: 39.1 pg/mL - 2500 pg/mL; Sample type: Cell culture supernatant, Cit plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | DKK1 |
|---|---|
| UniProt ID | O94907 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (By similarity). |
| Cellular Localization | Secreted. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
