Mouse DKK1 ELISA Kit(Dickkopf-1) (RMEK-0153699)
Cat. No.: RMEK-0153699
Category: ELISA Kits
INQUIRY
1 x 96 tests
DKK1 Mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse DKK1 in plasma and cell culture supernatants. (Mouse DKK1 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for mouse DKK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse DKK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This assay employs an antibody specific for mouse DKK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse DKK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | Multiple steps standard assay |
| Assay Type | Sandwich (quantitative) |
| Sensitivity | Sensitivity: 25 pg/mL |
| Range | 8.23 pg/mL - 6000 pg/mL |
| Sample Type | Cell culture supernatant, Plasma |
| Recovery | Cell culture supernatant-79.55-71% - 85%; Plasma-91.38-83% - 100% |
| Key Features | Sensitivity: 25 pg/mL; Range: 8.23 pg/mL - 6000 pg/mL; Sample type: Cell culture supernatant, Plasma; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | DKK1 |
|---|---|
| UniProt ID | O94907 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (By similarity). |
| Cellular Localization | Secreted. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
