Mouse DLL1 ELISA Kit (RMEK-0153700)
Cat. No.: RMEK-0153700
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse DLL1 ELISA Kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DLL1 protein in cell culture supernatant, serum, and plasma. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Mouse Serum-5-2.8%; Inter-Assay-Mouse Serum-3-7.2% |
| Sensitivity | 24 pg/mL |
| Range | 62.5 pg/mL - 4000 pg/mL |
| Sample Type | Cell culture supernatant, Plasma, Serum |
| Recovery | Cell culture supernatant-99-94% - 104%; Serum-100-99% - 101%; Hep Plasma-101-98% - 104%; EDTA Plasma-103-102% - 105%; Cit plasma-104-103% - 106% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 24 pg/mL; Range: 62.5 pg/mL - 4000 pg/mL; Sample type: Cell culture supernatant, Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | DLL1 |
|---|---|
| UniProt ID | O00548 |
| Biomarker of SCs/CSCs | Dukes Type C Colon Cancer |
| Function | Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. |
| Cellular Localization | Membrane. |
| Post-transcriptional Modifications | Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.