Mouse Fas Ligand ELISA Kit(FasL) (RMEK-0153727)
Cat. No.: RMEK-0153727
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse Fas Ligand ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fas Ligand protein in mouse serum, plasma and cell culture supernatant. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-3.9%; Inter-Assay-Serum-3-5.4% |
| Sensitivity | 5.7 pg/mL |
| Range | 18.75 pg/mL - 1200 pg/mL |
| Sample Type | Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Serum-105-98% - 121%; Cell culture media-101-96% - 104%; Hep Plasma-101-99% - 104%; EDTA Plasma-90-88% - 93%; Cit plasma-99-96% - 103% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 5.7 pg/mL; Range: 18.75 pg/mL - 1200 pg/mL; Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | FAS |
|---|---|
| UniProt ID | P25445 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. |
| Cellular Localization | Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface. |
| Post-transcriptional Modifications | N-glycosylated.The soluble form derives from the membrane form by proteolytic processing. |
| Involvement in Disease | Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B); also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
