Mouse FGFR4 ELISA Kit (RMEK-0153730)
Cat. No.: RMEK-0153730
Category: ELISA Kits
INQUIRY
1 x 96 tests
Mouse FGFR4 ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse FGFR4 protein in mouse serum, plasma, cell culture supernatant, and cell and tissue extract samples. The technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
Product Features
| Species Reactivity | Mouse |
|---|---|
| Detection Method | Colorimetric |
| Assay Duration | One step assay |
| Assay Time | 1.5 h |
| Assay Type | Sandwich (quantitative) |
| Precision | Intra-Assay-Serum-8-2.7%; Inter-Assay-Serum-3-3.4% |
| Sensitivity | 1.7 pg/mL |
| Range | 12.5 pg/mL - 800 pg/mL |
| Sample Type | Cell culture media, Cell Lysate, EDTA Plasma, Hep Plasma, Serum |
| Recovery | Serum-97-90% - 101%; Cell culture media-94-94% - 95%; Hep Plasma-82-80% - 85%; EDTA Plasma-87-84% - 91%; Cit plasma-99-96% - 101% |
| Key Features | One-wash 90 minute protocol; Sensitivity: 1.7 pg/mL; Range: 12.5 pg/mL - 800 pg/mL; Sample type: Cell culture media, Cell Lysate, EDTA Plasma, Hep Plasma, Serum; Detection method: Colorimetric; Assay type: Sandwich (quantitative); Reacts with: Mouse |
Target Information
| Target Symbol | FGFR4 |
|---|---|
| UniProt ID | P22455 |
| Biomarker of SCs/CSCs | Non Small Cell Lung Cancer |
| Function | Receptor for acidic fibroblast growth factor. Does not bind to basic fibroblast growth factor. Binds FGF19. |
| Cellular Localization | Membrane. Isoform 2 may be secreted. |
| Post-transcriptional Modifications | Glycosylated.Phosphorylated on tyrosine residue (By similarity). Phosphorylation requires the presence of a functional (phosphorylated) FGFR1 and not necessarily by means of FGFR heterodimerization. |
Storage & Shipping
| Storage | Store at 2-8°C |
|---|---|
| Shipping | Gel Packs |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. |
For research use only. Not for clinical use.
